Journal: Royal Society Open Science

Article Title: Symbiotic bacteria, immune-like sentinel cells, and the response to pathogens in a social amoeba

doi: 10.1098/rsos.230727

Figure Lengend Snippet: Paraburkholderia infection does not protect hosts from pathogenic Salmonella enterica (Se) or Staphylococcus aureus (Sa) bacteria. ( a ) Spore production of hosts when grown on food bacteria (F) or pathogenic Salmonella enterica (Se) or pathogenic Staphylococcus aureus (Sa). ( b ) Estimated effects (points) and 95% confidence intervals of Paraburkholderia infection (Pa, P. agricolaris ; Ph, P. hayleyella ), pathogen infection (Sa or Se) and co-infection (shown joined by *) on D. discoideum spore production. Spore production values were scaled by subtracting the mean and dividing by the standard deviation.

Article Snippet: To assess fitness effects associated with carriage of Paraburkholderia , we chose two pathogenic bacteria species known to infect D. discoideum intracellularly ( Staphylococcus aureus Rosenbach (Wichita) ATCC 29213, and Salmonella enterica ATCC 14028) [ , ].

Techniques: Infection, Bacteria, Standard Deviation

Journal: Nanomaterials

Article Title: Photocatalytic Inactivation of Plant Pathogenic Bacteria Using TiO 2 Nanoparticles Prepared Hydrothermally

doi: 10.3390/nano10091730

Figure Lengend Snippet: Antibacterial activity of A310C and P25 TiO 2 photocatalysts against Xanthomonas arboricola pv. juglandis , Allorhizobium vitis , Erwinia amylovora , and Pseudomonas syringae under UV-A irradiation. The concentration of aqueous TiO 2 dispersions was 0.5 mg/mL. Before UV-A irradiation, dispersions containing bacteria were kept in the dark for 15 min.

Article Snippet: For the pathogen inactivation study, four Gram-negative bacterium species such as the ATCC 49946 strain Erwinia amylovora (American Type Culture Collection, Manassas, VA, USA), the DSMZ 1049 strain Xanthomonas arboricola pv. juglandis (DSMZ-German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany), the DC3000 strain Pseudomonas syringae pv. tomato and the Allorhizobium vitis isolates were used.

Techniques: Activity Assay, Irradiation, Concentration Assay, Bacteria

Journal: Applied and Environmental Microbiology

Article Title: The Siderophore Product Ornibactin Is Required for the Bactericidal Activity of Burkholderia contaminans MS14

doi: 10.1128/AEM.00051-17

Figure Lengend Snippet: Antibacterial activity of Burkholderia contaminans MS14 against Xanthomonas citri pv. malvacearum MSCT1 (1), Pectobacterium carotovorum EC101 (2), Ralstonia solanacearum (3), Pseudomonas syringae pv. syringae B301D (4), Erwinia amylovora 2029 (5), Burkholderia glumae 291 (6), Escherichia coli (7), and Clavibacter michiganensis subsp. michiganensis Lu-01 (8). Aliquots (5 μl) of bacterial suspensions (optical density at 420 nm [OD420] values of 0.3) were inoculated onto the centers of NBY plates. After the plates had been incubated for 3 days at 28°C, the NBY plates were oversprayed with a suspension of indicator pathogenic bacteria (OD420 values of 0.3). Inhibition zones were measured from the margins of bacterial colonies 24 h later. The results show that MS14 has broad-spectrum antibacterial activities against the tested pathogens.

Article Snippet: Using this reference, the ornibactin loci were identified for 13 Burkholderia species, including the pathogenic species Burkholderia multivorans ATCC 17616, Burkholderia mallei ATCC 23344, Burkholderia thailandensis E264, Burkholderia oklahomensis EO147, Burkholderia pseudomallei 1026b, and B. pseudomallei K96243 ( 29 , – 32 ), the PGPB Burkholderia lata 383, Burkholderia ambifaria AMMD, and Burkholderia phytofirmans PsJN ( 33 , – 35 ), and the soil isolates B. cepacia GG4, Burkholderia vietnamiensis G4, Burkholderia phymatum STM815, and Burkholderia xenovorans LB400 ( 36 , – 38 ).

Techniques: Activity Assay, Incubation, Suspension, Bacteria, Inhibition

Journal: Applied and Environmental Microbiology

Article Title: The Siderophore Product Ornibactin Is Required for the Bactericidal Activity of Burkholderia contaminans MS14

doi: 10.1128/AEM.00051-17

Figure Lengend Snippet: Antibacterial activities of Burkholderia contaminans MS14

Article Snippet: Using this reference, the ornibactin loci were identified for 13 Burkholderia species, including the pathogenic species Burkholderia multivorans ATCC 17616, Burkholderia mallei ATCC 23344, Burkholderia thailandensis E264, Burkholderia oklahomensis EO147, Burkholderia pseudomallei 1026b, and B. pseudomallei K96243 ( 29 , – 32 ), the PGPB Burkholderia lata 383, Burkholderia ambifaria AMMD, and Burkholderia phytofirmans PsJN ( 33 , – 35 ), and the soil isolates B. cepacia GG4, Burkholderia vietnamiensis G4, Burkholderia phymatum STM815, and Burkholderia xenovorans LB400 ( 36 , – 38 ).

Techniques: Inhibition

Journal: Applied and Environmental Microbiology

Article Title: The Siderophore Product Ornibactin Is Required for the Bactericidal Activity of Burkholderia contaminans MS14

doi: 10.1128/AEM.00051-17

Figure Lengend Snippet: Plate bioassays of antimicrobial activities of Burkholderia contaminans strain MS14 and the mutants MS14MT357 (luxR::Tn5) and MS14MT577 (orbI::Tn5). (A) Loss of antibacterial activity of strain MS14 against the bacterial indicator Erwinia amylovora in MS14MT357 and MS14MT577. (B) Antifungal activities of strains MS14, MT357, and MT577 against the fungal indicator Geotrichum candidum. The results show that the genes disrupted in the mutants are not related to production of the antifungal activity of strain MS14.

Article Snippet: Using this reference, the ornibactin loci were identified for 13 Burkholderia species, including the pathogenic species Burkholderia multivorans ATCC 17616, Burkholderia mallei ATCC 23344, Burkholderia thailandensis E264, Burkholderia oklahomensis EO147, Burkholderia pseudomallei 1026b, and B. pseudomallei K96243 ( 29 , – 32 ), the PGPB Burkholderia lata 383, Burkholderia ambifaria AMMD, and Burkholderia phytofirmans PsJN ( 33 , – 35 ), and the soil isolates B. cepacia GG4, Burkholderia vietnamiensis G4, Burkholderia phymatum STM815, and Burkholderia xenovorans LB400 ( 36 , – 38 ).

Techniques: Activity Assay

Journal: Applied and Environmental Microbiology

Article Title: The Siderophore Product Ornibactin Is Required for the Bactericidal Activity of Burkholderia contaminans MS14

doi: 10.1128/AEM.00051-17

Figure Lengend Snippet: Bacterial strains and plasmids

Article Snippet: Using this reference, the ornibactin loci were identified for 13 Burkholderia species, including the pathogenic species Burkholderia multivorans ATCC 17616, Burkholderia mallei ATCC 23344, Burkholderia thailandensis E264, Burkholderia oklahomensis EO147, Burkholderia pseudomallei 1026b, and B. pseudomallei K96243 ( 29 , – 32 ), the PGPB Burkholderia lata 383, Burkholderia ambifaria AMMD, and Burkholderia phytofirmans PsJN ( 33 , – 35 ), and the soil isolates B. cepacia GG4, Burkholderia vietnamiensis G4, Burkholderia phymatum STM815, and Burkholderia xenovorans LB400 ( 36 , – 38 ).

Techniques: Plasmid Preparation, Expressing

Journal: Applied and Environmental Microbiology

Article Title: The Siderophore Product Ornibactin Is Required for the Bactericidal Activity of Burkholderia contaminans MS14

doi: 10.1128/AEM.00051-17

Figure Lengend Snippet: Ornibactin biosynthesis locus genetics of Burkholderia species. The ornibactin loci showed a high degree of conservation of the orbL, orbJ, orbE, and pvdA genes among the Burkholderia species compared; those genes are responsible for the biosynthesis and secretion of ornibactin. The bar at the right bottom indicates the degrees of similarity (40 to 100%).

Article Snippet: Using this reference, the ornibactin loci were identified for 13 Burkholderia species, including the pathogenic species Burkholderia multivorans ATCC 17616, Burkholderia mallei ATCC 23344, Burkholderia thailandensis E264, Burkholderia oklahomensis EO147, Burkholderia pseudomallei 1026b, and B. pseudomallei K96243 ( 29 , – 32 ), the PGPB Burkholderia lata 383, Burkholderia ambifaria AMMD, and Burkholderia phytofirmans PsJN ( 33 , – 35 ), and the soil isolates B. cepacia GG4, Burkholderia vietnamiensis G4, Burkholderia phymatum STM815, and Burkholderia xenovorans LB400 ( 36 , – 38 ).

Techniques:

Journal: BMC Plant Biology

Article Title: LC-MS metabolomics profiling of Salvia aegyptiaca L. and S. lanigera Poir. with the antimicrobial properties of their extracts

doi: 10.1186/s12870-023-04341-5

Figure Lengend Snippet: The antimicrobial activities of different extracts of studied Salvia spp. against pathogenic strains. Data are expressed as diameter of inhibition zone (mm). E. extract: ethanol extract, W. extract: water extract, B. extract: benzene extract

Article Snippet: The antimicrobial activities of the ethanol, water and benzene extracts of the two studied species were tested against different pathogenic bacterial strains ( B. subtilis (ATCC 6633), P. aeruginosa (ATCC 90,274), E. coli (ATCC 8739) and S. aureus (ATCC 6538)) and fungal strains( C. albicans (ATCC 10,221) and M. reinelloids ) and compared with the standard antimicrobial drug (Gentamycin)which recorded as the most used antibiotic to treat several types of microbial infections [ ]; the results are shown in (Table ; Figs. and ).

Techniques: Inhibition, Control